Quantum chemical approaches to [NiFe] hydrogenase
نویسندگان
چکیده
منابع مشابه
[NiFe]-Hydrogenase maturation endopeptidase: structure and function.
Hydrogenase maturation endopeptidases catalyse the terminal step in the maturation of the large subunit of [NiFe]-hydrogenases. They remove a C-terminal extension from the precursor of the subunit, triggering a conformational switch that results in the bridging of the Fe and Ni atoms of the metal centre via the thiolate of a cysteine residue and in closure of the centre. This review summarizes ...
متن کامل[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation.
The [FeFe]- and [NiFe]-hydrogenases catalyze the formal interconversion between hydrogen and protons and electrons, possess characteristic non-protein ligands at their catalytic sites and thus share common mechanistic features. Despite the similarities between these two types of hydrogenases, they clearly have distinct evolutionary origins and likely emerged from different selective pressures. ...
متن کاملCrystal Structure Analysis of [NiFe] Hydrogenase Maturation Proteins
Introduction [NiFe]-hydrogenases harbor a complex metal cofactor, NiFe(CN)2CO, in their active sites. Its biosynthesis requires specific maturation machinery, in which six Hyp proteins (HypABCDEF) play key roles. Four Hyp proteins (HypCDEF) are involved in the biosynthesis and incorporation of the Fe(CN)2CO group. After Fe insertion, HypA and HypB insert the Ni ion into the hydrogenase large su...
متن کاملHybF, a zinc-containing protein involved in NiFe hydrogenase maturation.
HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the product was purified. This protein behaves like a monomer in gel filtration and c...
متن کاملInteraction between Hydrogenase Maturation Factors HypA and HypB Is Required for [NiFe]-Hydrogenase Maturation
The active site of [NiFe]-hydrogenase contains nickel and iron coordinated by cysteine residues, cyanide and carbon monoxide. Metal chaperone proteins HypA and HypB are required for the nickel insertion step of [NiFe]-hydrogenase maturation. How HypA and HypB work together to deliver nickel to the catalytic core remains elusive. Here we demonstrated that HypA and HypB from Archaeoglobus fulgidu...
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ژورنال
عنوان ژورنال: Essays in Biochemistry
سال: 2017
ISSN: 0071-1365,1744-1358
DOI: 10.1042/ebc20160079